We explore the possibility of characterizing sperm cells without the need to stain them using spectral and fluorescence lifetime analyses after multi-photon excitation in an insect model. The autofluorescence emission spectrum of sperm of the common bedbug, Cimex lectularius, was consistent with the presence of flavins and NAD(P)H. The mean fluorescence lifetimes showed smaller variation in sperm extracted from the male (tau m, τm = 1.54–1.84 ns) than in that extracted from the female sperm storage organ (tau m, τm = 1.26–2.00 ns). The fluorescence lifetime histograms revealed four peaks. These peaks (0.18, 0.92, 2.50 and 3.80 ns) suggest the presence of NAD(P)H and flavins and show that sperm metabolism can be characterized using fluorescence lifetime imaging. The difference in fluorescence lifetime variation between the sexes is consistent with the notion that female animals alter the metabolism of sperm cells during storage. It is not consistent, however, with the idea that sperm metabolism represents a sexually selected character that provides females with information about the male genotype. 相似文献
Abstract This paper examines economic growth in 52 African countries for 1961–2016 and seeks to find if there is common growth. As all African countries have their particular features, concerning climate, harvest, industry, size, politics, and infrastructure, and more, it seems best to rely on a non-parametric method. Dynamic Time Warping is such a convenient method, also as it allows leads and lags across countries to vary over time, and as it can easily be incorporated into a clustering technique. Five clusters are found, two of which concern Equatorial Guinea and Botswana, and the three other clusters have common growth rates of about 0, 2 and 4 over more than five decades. 相似文献
Journal of Chemical Ecology - Complex inter-organismal communication among plants, insects, and microbes in natural and agricultural ecological systems is typically governed by emitted and... 相似文献
Using large components made of nodular cast iron (GJS) in wind turbines enables the application of lightweight construction through the high degree of design freedom. Besides the sand-casting process, casting into a permanent metal mould, i.e. chill casting, leads to a finer microstructure and higher quasi-static mechanical properties as well as higher fatigue strength. Unfortunately, in present design methodologies specific fatigue data is only available for sand cast and not for chilled cast GJS. Thus, lightweight design strategies for large, chilled cast components are not achievable, which led to the publicly funded project “Gusswelle”. Based on material investigations of EN-GJS-400-18-LT chill cast, an optimized hollow rotor shaft is developed. The design process and the resulting shaft design are presented. The optimized hollow rotor shaft prototype will be tested on a full-scale test bench to validate the design methodology. The intended validation plan as well as the test bench setup is shown in this paper. Furthermore, the decreasing wall thickness influences the interference fit between main bearing and hollow rotor shaft. Thus, through the applied bending moment, inner ring creep is more probable to occur in the main bearing seat. The creeping behaviour is investigated with finite element simulations and a measuring method is presented.
Formaldehyde is an important chemical that is mostly handled in aqueous solutions, which generally also contain methanol; furthermore, also solutions of formaldehyde in other alcohols are used. The density of these solutions is an important thermophysical property. The available models of the density of formaldehyde-containing solutions, however, all have shortcomings, such as a poor accuracy or a limited range of applicability. Therefore, in the present work, a new model of the density in systems of the type (formaldehyde + water + alcohol) was developed. The alcohols that are presently included in the new model are methanol, 1-propanol, and isoprenol; an extension to other alcohols is straightforward. The model was developed using literature data and extensive new density data measured in this work covering binary, ternary, and quarternary solutions of formaldehyde in water, methanol, 1-propanol, and isoprenol at temperatures of K and formaldehyde concentrations of 0.06 − 0.30 g g−1. 相似文献
This paper describes a framework for assessment of the risk that a proposed machine or system, such as an aircraft or computer, will not operate to its required performance specifications when it is developed. The method is based upon decomposition of the system under assessment into a hierarchy of functionally or structurally defined assessment areas. Within each area, technical risks, and methods of assessing these risks, are identified. The framework provides a systematic structure for selecting assessment methods and integrating results of the use of selected methods into a coherent overall assessment of the system. 相似文献